Transcriptome+Metabolome

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The transcriptome is an important method to obtain gene expression in a
biological system, and the metabolome is the basis and direct readout of the
system’s phenotype. The metabolites are the final result of gene transcription
under internal and external regulation and are the material basis of the
observed phenotype. In the era of systems biology research, biological
processes and gene regulatory networks are complex and dynamic. It is often
insufficient to use a single dataset to study systems biology. Correlating
transcriptomic data that has a large number of differentially expressed genes
with differential metabolites detected by metabolomics can pinpoint key genes,
metabolites, and metabolic pathways that are closely associated with internal
changes in the system, and thereby explain biological problems in a more
holistic approach.

Technology Introduction of Transcriptomics and Metabolomics Services
Correlating transcriptomic data that has a large number of differentially
expressed genes with differential metabolites detected by metabolomics and
multi-omics can pinpoint key genes, metabolites, and metabolic pathways that
are closely associated with internal changes in the system, and thereby explain
biological problems in a more holistic approach.

Case Study: The Cardamine Enshiensis Genome Reveals Whole Genome Duplication
And Insight Into Selenium Hyperaccumulation And Tolerance (Huang Et Al., Cell
Discovery 2021)

To better understand the mechanisms of Se tolerance in Cardamine enshiensis,
the authors constructed the 443Mb genome for C. enshiensis and performed RNA-
seq and Widely-Targeted Metabolomics on seedlings treated with water (control)
or 400uM sodium selenite.

Mechanisms of Se tolerance and hyperaccumulation in C. enshiensis

In total, 29,671 differentially expressed genes were identified and a total of
558 metabolites were identified in the leaf tissue of C. enshiensis, of which
127 were differential metabolites. The authors found 10 flavone-related
metabolites were altered between the two groups, indicating that flavones play
a pivotal role in Se tolerance. KEGG analyses showed the significantly enriched
pathways were associated with the biosynthesis of secondary metabolites and
flavone/flavonoid/flavonol compounds. A Pearson’s correlation coefficient
threshold of r > 0.8 was used to identify the metabolites that were
significantly correlated with each gene and the results revealed that 175
transcripts were highly correlated (R2 > 0.96) with tricetin O-malonylhexoside
and amentoflavone.

If you want to know more about RNA-seq and metabolomics, please visit our
website.

As a preeminent metabolomics company with a well-equipped metabolomics
laboratory, we provide cutting-edge metabolomics services through scientific
metabolomics test and analysis. As a preeminent metabolomics company with a
well-equipped metabolomics analysis laboratory, we provide cutting-edge
metabolomics services through scientific metabolomics test and analysis.

More details of metabolome project, please visit our website.

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