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Product Description Amp-future DNA Isothermal Amplification Kit NFO with 500-1000copies/µL Detection Limit, 48 Tests/box, -20°C Storage Product Detail Kit Storage and term of Validity Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing. Term of Validity: 14 months Isothermal nucleic acid Principle Summary The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3' end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method). Technical Parameters: Parameters Details Product Name DNA Isothermal Amplification Kit NFO Manufacturer Amp-future Storage Temperature -20°C Kit Components Enzymes, Buffers ,Reagents Packaging 48 Tests/box Detection Limit 500-1000copies/µL Shipping ICE Test Time 5-20mins Isothermal nucleic acid Product Features 1/ High sensitivity and specificity, short reaction time. 2/ The reagent form is freeze-dried, stable and easy to operate. 3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus. Isothermal nucleic acid Applications Suitable for DNA isothermal rapid amplification kit(NFO type) Primer: Require pair of nucleotide primers with the length of 25-35 bp. DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes. Notes 1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit. 2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

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