Rat Alpha Amylase ELISA kit

E02A0010 Rat Alpha Amylase ELISA kit

Rat Alpha Amylase ELISA kit is suitable for the detection of samples from Rat
species. Alpha Amylase can also be called glycogenase, α amylase, endoamylase,
Taka-amylase A, 1,4-α-D-glucan glucanohydrolase, AMY.

Principle of the Assay

AMY ELISA kit applies the competitive enzyme immunoassay technique utilizing an
anti-AMY antibody and an AMY-HRP conjugate. The assay sample and buffer are
incubated together with AMY-HRP conjugate in pre-coated plate for one hour.
After the incubation period, the wells are decanted and washed five times. The
wells are then incubated with a substrate for HRP enzyme. The product of the
enzyme-substrate reaction forms a blue colored complex. Finally, a stop
solution is added to stop the reaction, which will then turn the solution
yellow. The intensity of color is measured spectrophotometrically at 450nm in a
microplate reader. The intensity of the color is inversely proportional to the
AMY concentration since AMY from samples and AMY-HRP conjugate compete for the
anti-AMY antibody binding site. Since the number of sites is limited, as more
sites are occupied by AMY from the sample, fewer sites are left to bind AMY-HRP
conjugate. A standard curve is plotted relating the intensity of the color
(O.D.) to the concentration of standards. The AMY concentration in each sample
is interpolated from this standard curve.

Citations of Rat Alpha Amylase ELISA kit
E02A0010 has been referenced in the below publications:

The protective effect and mechanism of the alanyl-glutamine dipeptide in the
treatment of severe acute pancreatitis.

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