Rat Active Caspase 3 ELISA kit

E02A0015 Rat Active Caspase 3 ELISA kit

Rat caspase 3 elisa kit is suitable for the detection of samples from Rat
species. Active caspase 3 can also be called CPP32, CPP32B, SCA1, Apoptain,
Yama, Apoptosis-Related Cysteine Peptidase, Cysteinyl Aspartate Specific
Proteinases 3, SREBP cleavage activity 1, Active casp3, casp3, casp-3, casp 3.

Principle of the Assay

ACTIVE CASPASE 3 ELISA kit applies the quantitative sandwich enzyme immunoassay
technique. The microtiter plate has been pre-coated with a monoclonal antibody
specific for ACTIVE CASPASE 3. Standards or samples are then added to the
microtiter plate wells and ACTIVE CASPASE 3 if present, will bind to the
antibody pre-coated wells. In order to quantitatively determine the amount of
ACTIVE CASPASE 3 present in the sample, a standardized preparation of
horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for
ACTIVE CASPASE 3 are added to each well to “sandwich” the ACTIVE CASPASE 3
immobilized on the plate. The microtiter plate undergoes incubation, and then
the wells are thoroughly washed to remove all unbound components. Next,
substrate solutions are added to each well. The enzyme (HRP) and substrate are
allowed to react over a short incubation period. Only those wells that contain
ACTIVE CASPASE 3 and enzyme-conjugated antibody will exhibit a change in color.
The enzyme-substrate reaction is terminated by addition of a sulphuric acid
solution and the color change is measured spectrophotometrically at a
wavelength of 450 nm. A standard curve is plotted relating the intensity of the
color (O.D.) to the concentration of standards. The ACTIVE CASPASE 3
concentration in each sample is interpolated from this standard curve.

Citations of Rat Active caspase 3 ELISA kit
E02A0015 has been referenced in the below publications:

Hypoxic Preconditioning Augments the Therapeutic Efficacy of Bone Marrow
Stromal Cells in a Rat Ischemic Stroke Model.

Shanghai Bluegene Biotech has been committed to R&D of ELISA kits, making our
ELISA kits more convenient for researchers to use on the premise of
guaranteeing the quality, thus assisting scientific research for our customers.
So far, we have received 1200 published papers from researchers with a total of
about 500 kinds of ELISA Kits that have been cited. Bluegene ELISA kit has the
advantages of high specificity, high sensitivity, and high stability.

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