Proteomics Analysis Service

The term "proteomics" is the combination of "protein" and "genomics",
referring to the full set of proteins expressed by a genome, which consists
of all the proteins expressed by a cell or an organism. In 1995 (though
there are mentions of 1994 and 1996), Marc Wilkins first proposed the
concept of proteome, and in 1997, Peter James (who worked at the Swiss
Federal Institute of Technology, known as the European MIT) further
introduced the concept of proteomics. The concepts of genomics and
proteomics have further given rise to various other omics, and their birth
and development have also made systematic biology possible.

Research on proteome not only provides a material basis for understanding
the laws of life activities but also offers theoretical basis and solutions
for elucidating numerous disease mechanisms. By comparing the proteome of
normal and pathological individuals, we can identify certain disease-
specific protein molecules, which may serve as molecular targets for new
drug design, or may also provide molecular markers for early disease
diagnosis. Indeed, some of the best-selling drugs worldwide are either
proteins or target-specific protein. Thus, proteomics research is not only
essential for exploring the mysteries of life but also benificial to human
health. The study of proteomics is a characteristic of life sciences
entering the post-genomic era.

Proteomics refers to the systematic identification and quantification of the
proteome of a biological system (cell, tissue, organ, body fluid, or
organism) at a specific time point. Mass spectrometry (MS) is the most
commonly used technique for proteomics. MtoZ Biolabs, based on high-
throughput mass spectrometry technology, has established many proteomics
analysis platforms, possessing first-class MS-based proteomics techniques
and analysis capabilities, to provide a complete set of proteomics
solutions.

1. Protein Identification
Protein identification is a fundamental step in proteomics, aimed at
identifying the proteins in a sample, and is critical for determining the
nature of protein products. The molecular weight, isoelectric point,
structure, and amino acid sequence of a protein are key attributes in
determining the type of protein. MtoZ Biolabs possesses high-resolution mass
spectrometry, capillary electrophoresis, and circular dichroism methods for
protein identification, enabling precise identification of protein by
detecting these attributes and meeting various research needs. Whether your
sample is protein mixture, SDS-PAGE protein bands, 2D protein gel spots, or
pull-down and Co-IP samples, we can provide efficient and accurate
identification.

2. Quantitative Proteomics Analysis
Protein quantification is the process of determining the relative or
absolute quantity of proteins in samples. In proteomics research,
researchers often need to compare protein expression levels under different
conditions, such as normal versus disease states, or research changes of
protein expression levels over different time points. Quantification of
protein can be achieved through various methods, such as isotopic labeling
(e.g., TMT, iTRAQ, SILAC, 15N labeling) and label-free quantification,
providing information on protein quantities in different biological samples.
MtoZ Biolabs quantitative proteomics platform offers various protein
quantification techniques, including Label-free, iTRAQ, TMT, SILAC, 2D-DIGE,
DIA, SWATH, and relative, semi-quantitative, and absolute quantification to
meet various research needs, and free consultation is available.

3. Protein Sequencing
Protein sequencing involves analyzing the sequence of amino acid residues
that make up the primary structure of protein. Protein sequencing is
essential for understanding the structure and biological functions of
proteins within organism. MtoZ Biolabs uses nano LC-MS/MS nanoscale
chromatography combined with tandem mass spectrometry and Shimadzu's Edman
degradation sequencing system to analyze protein sequences, offering MS-
based protein sequencing analysis service, including analysis of protein
amino acid composition, N-terminal sequencing, C-terminal sequencing, and
full-length sequence, as well as Edman degradation-based N-terminal protein
sequence analysis. For proteins with unknown theoretical sequences, we
provide de novo protein sequencing services to analyze the protein sequence.

4. Post-Translational Modifications Proteomics Analysis
Post-translational modifications (PTMs) refer to the chemical modifications
that occur in proteins after its translation, which can affect the function,
stability, and localization of proteins. Common PTMs include protein
phosphorylation, protein glycosylation, protein ubiquitination, protein
methylation, protein acetylation, etc. The study of PTMs requires high-
resolution mass spectrometry and other technologies to identify and quantify
different forms of modified proteins. MtoZ Biolabs has advanced analysis
platforms and various enrichment methods, capable of identifying low-
abundance modified peptides and characterizing various types of PTMs,
including whether modifications occur, what type of modifications occur,
which amino acid sites undergoes modifications, and how many sites undergoes
modifications.

MtoZ Biolabs has much experience in proteomics service with a comprehensive
quality control workflow and experienced technical personnel capable of
handling various types of samples. We can provide you with a one-stop
proteomics service and can tailor project plans based on actual research
needs. You only need to tell us your experimental objectives and send us
your samples, MtoZ Biolabs will take care of all subsequent matters. More
proteomics services are available for free consultation.

About Us

MtoZ Biolabs is an integrate contract research organization (CRO) providing
advanced proteomics, metabolomics, bioinformatics, and biopharmaceutical
analysis services to researchers in biochemistry, biotechnology, and
biopharmaceutical fields. The name of MtoZ represents “mass to charge
ratio” in mass spectrometry analysis, as most of our services are provided
based on our well-established mass spectrometry platforms. Our services
allow for the rapid and efficient development of research projects,
including protein analysis, proteomics, and metabolomics programs.

MtoZ Biolabs is specialized in quantitative multiplexed proteomics and
metabolomics applications through the establishment of state-of-the-art mass
spectrometry platforms, coupled with high-performance liquid chromatography
technology. We are committed to developing efficient, and effective tools
for addressing core bioinformatics problems. With a continuing focus on
quality, MtoZ Biolabs is well equipped to help you with your needs in
proteomics, metabolomics, bioinformatics, and biopharmaceutical research.
Our ultimate aim is to provide more rapid, high-throughput, and cost-
effective analysis, with exceptional data quality and minimal sample.

Email: marketing@mtoz-biolabs.com

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