PakGent 40ul 384-Well PCR Plate

PCR Tubes with 40ul 384-Well PCR Plate are a type of labware used in PCR.
The PCR tubes are manufactured from high-quality polypropylene and are
designed to fit into a 384-well PCR plate. Each tube has a total capacity of
40ul and a lid that screws on and off for easy sample access. The qpcr 384
well plate provides an optimal reaction chamber for the PCR process,
allowing for uniform and efficient amplification. The PCR tubes are
compatible with most automated thermal cyclers and can be used for a variety
of PCR applications such as genotyping, gene expression, and DNA sequencing.

Parameter of 40ul 384-Well PCR Plate


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Improving PCR Efficiency in 384-Well Plates
1. Use smaller reaction volumes: Using smaller reaction volumes in 384-well
plates can improve PCR efficiency by increasing the reaction surface area
and reducing the amount of reagents used. This can also reduce the risk of
contamination due to the smaller reaction volumes.
2. Minimize the distance between the reaction and the thermal cycler block:
Increasing the distance between the reaction and the thermal cycler block
can reduce the time needed to reach the desired temperature and therefore
improve PCR efficiency.
3. Use optimized reaction conditions: Optimizing the reaction conditions,
such as the amount of primer and template, the concentration of MgCl2 and
the reaction temperature, can improve the PCR efficiency in 384-well plates.
4. Use pre-dispensed reagents: Pre-dispensing the PCR reagents into the
wells of the 384-well plate can reduce the risk of contamination and improve
the efficiency of the PCR reaction.
5. Use high-quality reagents: Using high-quality reagents can improve the
efficiency of the PCR reaction by reducing the risk of contamination and
ensuring that the reaction conditions are optimal.
6. Use improved thermal cyclers: Using improved thermal cyclers with a
faster ramping rate and a more accurate temperature control can improve the
efficiency of the PCR reaction in 384-well plates.

Troubleshooting Guide for PCR Amplification Issues with the 40ul 384-Well
PCR Plate
1. No amplification signal:
- Verify thermal cycler settings, PCR reagents, and proper sealing of the
PCR plate.
- Adjust annealing temperature and extension time if needed.
2. Weak or low amplification signal:
- Check DNA quality and concentration.
- Optimize primer concentration and design.
- Increase the cycling cycles.
3. Non-specific amplification:
- Optimize annealing temperature and primer design.
- Consider touchdown or nested PCR protocols.
- Check for contamination in PCR reagents.
4. Noisy background or smearing:
- Ensure DNA template, primers, and PCR reagents are contamination-free.
- Optimize PCR conditions.
- Maintain a clean workspace.
5. Incomplete or partial amplification:
- Check DNA template quality and concentration.
- Verify primer specificity and adjust PCR conditions.
- Consider using more efficient DNA polymerase or enhancers.
If issues persist, consult technical support or the PakGent's
troubleshooting guide.

Features and Benefits of the PakGent 40ul 384-Well PCR Plate
The PakGent 40ul 384-Well PCR Plate is a high-quality labware designed for
PCR applications. Its features include a durable polypropylene construction,
accurate 40ul capacity per tube, compatibility with 384-well plate format,
screw lids for easy access, uniform amplification, versatility for various
PCR applications, compatibility with automated thermal cyclers, quality
assurance, convenient storage, and cost-effectiveness in high-throughput
workflows.

40ul 384-Well PCR Plate FAQs
01
Can these PCR tubes be used for qPCR?
Yes, these 384-Well PCR Plate tubes can be used for quantitative PCR (qPCR)
applications. They are designed to withstand the thermal cycling and
fluorescence detection requirements of qPCR assays.
02
What material are these PCR tubes made from?
These PCR tubes are made from high-quality polypropylene, which is a durable
and chemically resistant material commonly used in laboratory applications.
03
Are these PCR tubes compatible with automated thermal cyclers?
Yes, these PCR tubes are designed to be compatible with most automated
thermal cyclers commonly used in laboratories, allowing for seamless
integration into automated PCR workflows.
04
Can the PCR tubes be easily labeled for sample identification?
Yes, these pcr 384 well plate tubes usually have flat sides or frosted areas
that allow for easy labeling using markers or labels, ensuring proper
identification of samples during PCR experiments.

Product Certification

Factory Pictures

Minimum Order: 1 pounds

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