Liquid Chromogenic Culture Media

Liquid Chromogenic Culture Medium
Coliforms and E. coli liquid chromogenic medium
Performance Characteristics of Liquid Chromogenic Culture Media
1. It is included in the "Japan Food Hygiene Guide" (2004 edition) with strong
authoritative influence.
2. There are two kinds of chromogenic substances, which can directly recognize
coliforms and E. coli.
3. The test results can be obtained within 24 hours, but BGLB needs 48 hours.
4. No need for a fermentation tube and a constant temperature pool with low
cost and easy operation.
5. No need to prepare medium and wash tubes (except XM powder medium).
6. No need for identification and supplementation experiments such as EMB
culture.
7. E.coli O157 cannot be identified separately but qualitative testing only.
Product Details of Liquid Chromogenic Culture Media
Name Model Application Capacity Packing Validity Period
Liquid chromogenic culture medium XM-30 For 1ml sample inspection
10ml/cup 128 cups/carton 1 year
Liquid chromogenic culture medium XM-31 For 10ml sample inspection
10ml/cup 128 cups/carton
Liquid chromogenic culture medium XM-32 For 5ml sample inspection
5ml/cup 128 cups/carton
XM powder medium XM0001 For preparing medium 8.5g/bag 20
bags/carton 3 years
MT opener OS-01 For MT container opening / 1pc /
Usage Method of Liquid Chromogenic Culture Media
1. Container surface sterilization: The surface of the aluminum foil should be
disinfected with an alcohol lamp before use.
2. Opening: Open the aluminum foil with an MT opener (OS-01) or open directly
with a sterile pipette.
3. Inject the sample diluent: use a pipette to inject the sample dilution into
the cup
XM-30: 1ml, XM-31: 10ml, XM-32: 5ml
4. Cultivation: Culture time: 24 hours; Culture temperature: 36 ± 1 °C
5. Disposal: Dispose of them after sterilization
※XM powder medium usage: Dissolve 500ml of pure water per bag, inject 10ml
into the test tube, autoclave, then inject 1ml sample, and incubate at 36±1°C
for 24 hours. The identification method is the same.
Coloring Principle
Coliforms positive
There is a special β-galactosidase only exit in the coliforms. It can be used
as an indicator enzyme for coliform bacteria, which catalyzes the hydrolysis of
the substrate X-GAL to form 5,5-dibromo -4,4-dichloro indigo, appearing light
blue ~ purple-blue.
E. coli positive
β--glucuronidase can be used as an indicator enzyme for Escherichia coli;
because β-glucuronidase can be observed in Escherichia coli, Salmonella and
Shigella in intestinal bacteria, and 95% of E. coli contains β-glucuronidase.
It catalyzes the hydrolysis of MUG to form 4-methylumbelliferon, which exhibits
fluorescence at 366 nm ultraviolet light and is visually discernible under
indoor light intensity.
Operation and Coloring of Liquid Chromogenic Culture Media
ELEX Biological Products (Shanghai) Co., Ltd., established in 2013, is a colony
counter manufacturer, which specializes in sales, research and development of
microbiological test products for food hygiene testing and environment testing,
simultaneously act as an agency of microbial testing equipment from ELMEX
Corporation of Japan. The factory has passed ISO9001:2015, and the products
have passed the strict assessment of the quality management of the Japanese
food industry and are favored by the industry. Its top popular product---ST
swab sampler series account for 70% of the market rate in Japan.

Minimum Order: 100000 pieces

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