Human Total β Amyloid Protein ELISA kit

E01A0039 Human Total β Amyloid Protein ELISA kit

Human Total β Amyloid Beta ELISA kit is suitable for the detection of samples
from human species. Total β Amyloid Protein can also be called Aβ, Aβ
protein, Amyloid beta-peptide, Beta-amyloid peptide, Beta-amyloid protein, beta
Amyloid Protein.

Specifications of Human Total β Amyloid Protein Bluegene ELISA kit
Product Information
Cat. No. E01A0039
Product Name Human Alkaline Phosphatase ELISA kit
Species Human
Product Size 48 Tests / 96 Tests
Concentration 5.0-100ng/mL
Sensitivity 1.0 ng/mL
Principal Sandwich ELISA
Sample Volume 50 ul
Sample Type Serum, plasma, cell culture supernatants, body fluid and tissue
homogenate
Assay Time 90 minutes
Platform Microplate Reader
Conjugate HRP
Detection Method Colorimetric
Storage 2-8°C

Principle of the Assay

AΒ ELISA kit applies the quantitative sandwich enzyme immunoassay technique.
The microtiter plate has been pre-coated with a monoclonal antibody specific
for AΒ. Standards or samples are then added to the microtiter plate wells and
AΒ if present, will bind to the antibody pre-coated wells. In order to
quantitatively determine the amount of AΒ present in the sample, a
standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal
antibody, specific for AΒ are added to each well to “sandwich” the AΒ
immobilized on the plate. The microtiter plate undergoes incubation, and then
the wells are thoroughly washed to remove all unbound components. Next,
substrate solutions are added to each well. The enzyme (HRP) and substrate are
allowed to react over a short incubation period. Only those wells that contain
AΒ and enzyme-conjugated antibody will exhibit a change in color. The enzyme-
substrate reaction is terminated by addition of a sulphuric acid solution and
the color change is measured spectrophotometrically at a wavelength of 450 nm.
A standard curve is plotted relating the intensity of the color (O.D.) to the
concentration of standards. The AΒ concentration in each sample is
interpolated from this standard curve.


Citations of Human Total β Amyloid Protein ELISA kit
E01A0039 has been referenced in the below publications:

The study of the Characteristics of Cognitive Impairment after Acute Mild-to-
Moderate Ischemic Stroke and the Role of SAA and Aβ in Cognitive Impairment.

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