Human Adenosine Triphosphate ELISA kit

E01A0038 Human Adenosine Triphosphate ELISA kit

Human Adenosine Triphosphate ATP ELISA kit is suitable for the detection of
samples from human species. Adenosine Triphosphate can also be called
Adenosine-5'-Triphosphate, ATP.

Specifications of Human Adenosine Triphosphate ATP ELISA kit
Product Information
Cat. No. E01A0038
Product Name Human Adenosine Triphosphate ELISA kit
Species Human
Product Size 48 Tests / 96 Tests
Concentration 50-1000 ng/mL
Sensitivity 1.0 ng/mL
Principal Competitive ELISA
Sample Volume 100 ul
Sample Type Serum, plasma, cell culture supernatants, body fluid and tissue
homogenate
Assay Time 90 minutes
Platform Microplate Reader
Conjugate HRP
Detection Method Colorimetric
Storage 2-8°C

Principle of the Assay

ATP ELISA kit applies the competitive enzyme immunoassay technique utilizing an
anti-ATP antibody and an ATP-HRP conjugate. The assay sample and buffer are
incubated together with ATP-HRP conjugate in pre-coated plate for one hour.
After the incubation period, the wells are decanted and washed five times. The
wells are then incubated with a substrate for HRP enzyme. The product of the
enzyme-substrate reaction forms a blue colored complex. Finally, a stop
solution is added to stop the reaction, which will then turn the solution
yellow. The intensity of color is measured spectrophotometrically at 450nm in a
microplate reader. The intensity of the color is inversely proportional to the
ATP concentration since ATP from samples and ATP-HRP conjugate compete for the
anti-ATP antibody binding site. Since the number of sites is limited, as more
sites are occupied by ATP from the sample, fewer sites are left to bind ATP-HRP
conjugate. A standard curve is plotted relating the intensity of the color
(O.D.) to the concentration of standards. The ATP concentration in each sample
is interpolated from this standard curve.

Quality Control on Human Adenosine Triphosphate ELISA kit
Coefficient of Variance Intra Variation% ＜10%
Inter Variation% ＜12%
Recovery 94-106%
Linearity Diluent Ratio Range %
1:02 93-101
1:04 90-107
1:08 92-105
Specificity/Cross-reactivity No significant cross-reactivity or interference
between ATP and analogues was observed.

Citations of Human Adenosine Triphosphate ELISA kit
E01A0038 has been referenced in the below publications:

The research of the metabolism and in vivo functional changes of erythrocyte
stored at -65℃.

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