DNA Isothermal Amplification Kit NFO With 500-1000 Copies/UL Detection Limi

Product Description
Amp-future DNA Isothermal Amplification Kit NFO with 500-1000copies/µL
Detection Limit, 48 Tests/box, -20°C Storage
Product Detail
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and
repeated freezing and thawing.

Term of Validity: 14 months



Isothermal nucleic acid Principle Summary
The kit is based on room and constant temperature nucleic acid rapid
amplification technology, its principle is that at room and constant
temperature, the recombinase and primer form a protein/single-stranded
nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template
with the help of auxiliary proteins and single-stranded binding protein SSB;
then form a D-loop region at the invasion point and start to scan the DNA
duplex, after finding the target region complementary to the primer and
disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3'
end of the primer to start the chain extension. The kit relies on the role
of NFO enzyme and adds the designed specific molecular probes according to
the template, and get the result by colloidal gold technology (sandwich
method).



Technical Parameters:
Parameters Details
Product Name DNA Isothermal Amplification Kit NFO
Manufacturer Amp-future
Storage Temperature -20°C
Kit Components Enzymes, Buffers ,Reagents
Packaging 48 Tests/box
Detection Limit 500-1000copies/µL
Shipping ICE
Test Time 5-20mins


Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without
purchasing expensive PCR apparatus.



Isothermal nucleic acid Applications
Suitable for DNA isothermal rapid amplification kit(NFO type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.



Notes
1/ Please avoid nucleic acid contamination and set blank control during
reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the
experiment, and put the rest under storage conditions when performing the
experiment.

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